Some standard methods are used to determine the lowest bactericidal concentrations of disinfectants and antiseptics. They generally investigate the effect of disinfectants on microorganisms in suspension. However, the results obtained with these tests do not give information about the effect of disinfectants on the shape of bacteria in the biofilm. However, bacteria that are important in many sectors, including health, are often found in biofilms and traditional disinfectant activity tests cannot detect their efficacy.

Therefore, studies are carried out to determine the lowest bactericidal concentrations of disinfectants commonly used in disinfection of surfaces and tools, materials and materials used in hospitals, health institutions and different sectors against static biofilm culture. Pseudomonas aeruginosa ATCC 15442 standard strain is used in these studies. This strain is a type of bacteria that is usually found in soil and water, which breaks down glucose by oxidation but does not ferment.

Bactericidal activity tests performed within the scope of disinfectant analyzes in accredited laboratories include basic bactericidal activity tests of disinfectants and antiseptics. In these tests, standards published by various domestic and foreign organizations are used. The standard comes first:

• TS EN 1276 Chemical disinfectants and antiseptics - Quantitative suspension test for the assessment of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, household and institutional areas - Test method and specifications

This standard describes a test method for determining the minimum properties of the bactericidal effect of homogenous and physically stable chemical disinfectant and antiseptic products when diluted with water in the case of hard water or ready-to-use products. Since a number of dilutions are generally produced by adding substances that disrupt the microorganism being tested, the products are only tested at a density of 80 or less.