In some cases, the main causes of poisoning in humans by consuming sea water, shellfish and various fish are Vibrio bacteria. Vibrio bacteria start to show poisoning effect directly by themselves or toxins they secrete by consuming sea creatures obtained from polluted waters without paying attention to hygiene rules. Vibrio bacteria are gram negative, non-spore, motile and aerobic bacteria. In the laboratories, vibrio analysis is carried out either by direct sowing or by pre-enrichment and then by sowing. These analyzes are based on the following ISO standards as well as the standards published by the Turkish Standards Institute, which are shown below:

• ISO / TS 21872-1 Microbiology of food and animal feeds - Potentially enteropathogenic vibrio species - Part 1: Identification of vibrio parahaemolyticus and vibrio cholerae bacteria
• ISO / TS 21872-2 Microbiology of food and animal feed - Potentially enteropathogenic vibrio species - Part 2: Detection of bacterial species other than vibrio parahaemolyticus and vibrio cholerae

In advanced laboratories, studies are carried out for the determination of vibrio cholerae in food and animal feeds within the scope of microbiological analyzes. These studies are based on standards published by domestic and foreign organizations. The Turkish standards considered in this regard are:

• TS EN ISO 11348-1 Water quality - Determination of inhibition effects of water samples on light emissions of vibrio ficheri (luminescent bacteria test) - Part 1: by freshly cultivated bacteria method
• TS EN ISO 11348-2 Water quality - Determination of inhibition effects of water samples on light emissions of vibrio fischeris (luminescent bacteria test) - Part 2: by liquid drying method
• TS EN ISO 11348-3 Water quality - Determination of inhibition effects of water samples on light emissions of vibrio ficheri (Luminescent bacteria test) - Part 3: Freeze-dried bacteria method