In microbiology studies, when isolation is mentioned, it is meant to obtain a microorganism in pure form. In the natural environment, microorganisms are generally mixed and very rarely found in pure form. However, it is necessary to obtain pure cultures of microorganisms for various purposes. Pure culture is a collection of microorganisms grown from only one microorganism cell. For example, microorganisms used for industrial purposes, such as bacteria used in the production of dairy products, yeast and bacteria used in agricultural control, need to be used in pure form. In addition, in order to detect any deterioration or risk of disease in laboratory research, the microorganism must be in pure state.

One of the most important isolation practices in terms of food microbiology is salmonella, which is not allowed to be contained in foodstuffs by legal regulations, e. to detect the presence of pathogenic microorganisms such as coli and listeria. In laboratory studies, colonies of these bacteria are isolated in foods.

Briefly, isolation studies are performed to obtain only the desired microorganisms in pure form from a mixed culture or to check the purity of the microorganisms that are considered to be pure. Therefore, different working methods are used according to the purpose.

When identification is mentioned in microbiology studies, it is meant to determine the genus and species of an isolated microorganism. In addition to simple biochemical tests, advanced methods such as genetic, immunological or immunoenzymatic methods are used in these studies.

In the identification process, it is generally carried out in three stages. Morphological and physiological characteristics are determined first and then biochemical tests are performed. Then, serological and toxin determination analyzes are performed if necessary. Identification studies are demanding, demanding, time consuming and expensive applications.

In advanced laboratories, in the framework of microbiological analysis, bacillus spp. isolation and identification analysis. During these studies, the relevant legal regulations and the standards issued by domestic and foreign organizations are complied with.