In fact, there is no standard cultural analysis of total bacteria. Very special devices and analyzes are needed to count the total bacteria. As a standard analysis, the number of bacteria obtained in psychophile, mesophile and thermophile groups and in aerobic / anaerobic conditions is determined. Among these, analyzes are performed to determine the total number of aerobic mesophilic bacteria. Total aerobic mesophilic bacteria count is an easy and inexpensive quality standard that is very common for foods.

For total mesophilic aerobic bacteria count, colonies that develop in a solid medium at a certain temperature for a certain period of time and under favorable conditions are counted and the total number of aerobic mesophilic bacteria in foodstuffs is determined. In particular, psychophilic aerobic bacteria count is important for foodstuffs that are kept in the cold environment for a long time.

All kinds of foodstuffs offered for consumption in the world contain a certain amount of microorganisms. Only heat-treated pastes, canned foods and long-life milks are considered sterile, while food products such as carbonated soft drinks and fruit juices that are heat treated below one hundred degrees or less are considered semi-sterile.

Fecal coliform bacteria and pathogenic microorganisms are not allowed in foodstuffs in accordance with the relevant standards and regulations. The total number of aerobic mesophilic bacteria, that is, the number of microorganisms capable of multiplying at normal temperature of the body in an oxygenated environment, is allowed to be up to a certain limit.

Determination of the total number of bacteria in foodstuffs is widely used to determine the microbiological quality. If the total number of viable bacteria in food products is high, this is an expression of bacteria that cause disease in these foods.

Within the framework of microbiological analyzes, studies are carried out to determine the number of aerobic bacteria, number of aerobic mesophilic bacteria or number of aerobic colonies in foodstuffs in authorized laboratories. During these studies, relevant legal regulations and standards are complied with.