fumonisin (B1 + B2) determination (HPLC ve LC-MS / MS)

Fumonisin toxins are among the most recently discovered among the toxic mycotoxins produced by mold microorganisms. Produced by molds of Fusarium species. These molds are mostly found in the corn plant and spread to the nature during corn harvesting. Among the fumonisin toxins, B1, B2 and B3, fumonisin B1 is the most common in nature.
In animal tests, fumonis caused brain damage in horses, lung edema in pigs and liver cancer in mice. Although not fully identified, this toxin is also considered to be dangerous to human health. It is known that up to now it has caused liver cancer in some parts of China and there have been cases of laryngeal cancer in South Africa. Therefore, the International Agency for Research on Cancer has recognized that fumonisin is a potential cancer risk for humans and is listed as a carcinogen.
Fumonisin B1 is a poison that is resistant to most normal food preparation processes. It is soluble in water and in many polar solvents. There is no known disinfection method for fumonycin-contaminated foods and animal feeds. With grinding and the application of ammonia, the level of fumonisin decreases slightly.
The permissible limit values for fumonisins are shown in the annexes of Turkish Food Codex Contaminants Regulation published by the Ministry of Food, Agriculture and Livestock. According to this, fThe total umonisin (B1 + B2) values in μg / kg are as follows:
- 4000 in unprocessed corn
- 1000 for corn and maize based products offered for direct consumption
- 800 for cookies in corn-based breakfast cereals
- 200 in processed corn-based infant and toddler supplements
- 500 in corn products produced by passing through sieves larger than 1400
- 500 in corn products produced by passing through sieves smaller than 2000
Within the scope of mycotoxin analysis in advanced laboratories, HPLC and LC-MS / MS methods are applied to determine the fumonisin (B1 + B2) studies. During these studies, standards published by domestic and foreign organizations are based.