Diasetoksisirperol (Diacetoxyscirpenol) determination (HPLC ve LC-MS / MS)

Determination of Diacetoxysirperol (Diacetoxyscirpenol) (HPLC and LC-MS / MS)
Determination of Diacetoxysirperol (Diacetoxyscirpenol) (HPLC and LC-MS / MS)

Mycotoxins are secondary metabolites secreted by molds and cause poisoning in humans and animals. For the formation of mycotoxins, a number of physical, chemical and biological factors must be brought together. Humidity, temperature, drying rate of the product, re-wetting, grinding methods of grains, time and mechanical damages are among the physical factors. The level of oxygen and carbon dioxide in the air, the type and properties of foodstuffs and whether any chemical treatment is applied are among the chemical factors. Biological factors are plant species, plant damage, insects and most importantly molds in the environment.

According to the following regulations issued by the Ministry of Food, Agriculture and Livestock, the highest values ​​of certain contaminants, which can be found in foods, according to mycotoxin species, are explained:

·         Turkish Food Codex Contaminants Regulation

·         Turkish Food Codex Communiqué on Maximum Limits of Contaminants in Foodstuffs (Communiqué no: 2008 / 26)

One of the most important mycotoxins produced by the fungi of the Fusarium species, generally found in cereals, is tirkothecenes. The most common trichothecenes include diacetoxycirperol, T2 toxin, and vomitoxin (or deoxynivalenol). Diacetoxysirperol species are mostly found in rice, barley, wheat, corn, oats and rye.

Various analysis methods are used to determine the presence and amount of diacetoxysirperol. High performance liquid chromatography using HPLC is one of these methods. This method uses analytical separation techniques. Another method is the liquid chromatography - mass - mass spectrometer method called LC-MS / MS. In this method, sample molecules are separated according to their physicochemical properties and analyzed by mass detector with the help of a special device.

Within the scope of mycotoxin analysis in advanced laboratories, HPLC and LC-MS / MS methods are applied to determine the diacetoxysirperol (diacetoxyscirpenol). These analyzes are based on standards published by domestic and foreign organizations.