Deoxynivalenol (DON) is also known as vomitoxin and is a type of mycotoxin produced by fusarium molds found in cereal products. They are toxic and cause skin disorders and vomiting in humans, more importantly damaging the immune system. In animals, this compound causes excess weight loss. In the European Union countries, limits varying between 0,2 and 1,75 ppm are introduced. In animal feeds, these limit values ​​are between 0,9 and 12 ppm.

Since deoxynivalenols or vomitoxins are heat resistant between 170 and 350, it is not possible to destroy them by heat. Therefore, boiling, frying or cooking processes do not stop the activity of these mycoticins. Chemical spraying of grain products is not a preferred method because it damages the products and the risk of these products absorbing chemicals is very high. Therefore, generally applied ozone system is a very successful disinfection method to get rid of these compounds.

Various methods of analysis are used to determine the amount of deoxynivalenol or vomitoxin. One of these methods is a high performance liquid chromatography method using HPLC. This method is used for analytical separation techniques. It is a method with high sensitivity, quantitative results and easy to apply. It is preferred in the separation of non-volatile or easily degradable compounds in heat. Another method is the liquid chromatography - mass - mass spectrometer method called LC-MS / MS. This method is also a chemical analytical method. In cases where a rapid deoxynivalenol test result is needed, the ELISA kits give quantitative results within a maximum of 20 minutes.

Within the scope of mycotoxin analysis in advanced laboratories, HPLC and LC-MS / MS methods are applied to determine the amount of deoxynivalenol or vomitoxin. These analyzes are based on standards published by domestic and foreign organizations.