aflatoxin M1 determination (HPLC ve LC-MS / MS)

Aflatoxin M1 Determination (HPLC and LC-MS / MS)
Aflatoxin M1 Determination (HPLC and LC-MS / MS)

Mycotoxins, known as mold poison, produce a poison that is harmful to human and animal health and the most important of them are aflatoxins. Among the aflatoxins, the most toxic is aflatoxin B1, which has cancer effect and has an effect on gene structure. Until recently, aflatoxin M1, aflatoxin D2, aflatoxin P1 and aflatoxin Q2 have been identified as a result of studies on aflatoxins known to four species (B1, B1, G1 and G1). These types of aflatoxins result from the metabolism of aflatoxin B1 in the liver of animals. In the studies, the milk toxins which are excreted with the milk of the animals are named aflatoxin M1 and aflatoxin M2. It is mostly seen in milk from cows fed with aflatoxin B1. If one liter of milk contains an amount of aflatoxin M0,05 over 1 micrograms, this leads to jaundice and serious damage to the liver. Aflatoxins are carcinogenic and highly toxic compounds.

The most important sources of aflatoxins are feedstuffs or molds that are prepared or stored under inappropriate conditions. When animals feed on these feeds, mycotoxins pass to milk. An important point is that the aflatoxin M1 compound is resistant to pasteurization processes. Therefore, it should be checked not only in raw milk but also in processed milk runes.

Different analysis methods are used to determine the amount of aflatoxin M1. One of these methods is high performance liquid chromatography (HPLC). The HPLC device is used for analytical separation techniques. Another method used in the analysis is liquid chromatography - mass - mass spectrometry (LC-MS / MS). This method is also a chemical analytical method.

Within the scope of mycotoxin analysis in advanced laboratories, studies are carried out to determine the amount of aflatoxin M1 by HPLC and LC-MS / MS methods. These analyzes are based on standards published by domestic and foreign organizations.