aflatoxin B1 ve Total aflatoxin determination (B1 B2 + + + G1 G2) (HPLC ve LC-MS / MS)

Determination of Aflatoxin B1 and Total Aflatoxin (B1 + B2 + G1 + G2) (HPLC and LC-MS / MS)
Determination of Aflatoxin B1 and Total Aflatoxin (B1 + B2 + G1 + G2) (HPLC and LC-MS / MS)

Until recently, four species were known in chromotographic studies on aflatoxins. Two of them give blue fluorescence under ultraviolet rays and are named aflatoxin B1 and aflatoxin B2 according to their color. The other two give green fluorescence and are named aflatoxin G1 and aflatoxin G2 according to their color (B is blue and G is green). Subsequently, the milk toxins excreted in the milk of the animals during the studies carried out were named aflatoxin M1 and aflatoxin M2 (abbreviation M milk). Aflatoxin B1 is metabolized in the liver of animals and becomes less active poisons such as aflatoxin M1, aflatoxin D1, aflatoxin P1 and aflatoxin Q1.

Due to its toxic properties, a limit has been set for the amount of aflatoxin B1 to be found in foodstuffs in European Union countries. This compound can contain at most 5 ppb in milliliters, 10 ppb in animal feeds and 0,5 ppb in milk. In our country, the highest permitted aflatoxin B1 value for certain foodstuffs is 5 ppb and total aflatoxin (B1 + B2 + G1 + G2) is 10 ppb.

Different analysis methods are used to determine the amount of aflatoxin B1 and total aflatoxin (B1 + B2 + G1 + G2). One of these is the high performance liquid chromatography method (HPLC). In the analysis made by HPLC device, mobile phase is given at high pressure and high resolution separation is provided. Another method of analysis is liquid chromatography - mass - mass spectrometry (LC-MS / MS). This method is a chemical analytical method and combines the mass analysis capabilities of the mass spectrometer with the physical separation properties of liquid chromatography.

Within the scope of mycotoxin analysis in advanced laboratories, studies are conducted to determine the amounts of aflatoxin B1 and total aflatoxin (B1 + B2 + G1 + G2) using HPLC and LC-MS / MS methods. These analyzes are based on standards published by domestic and foreign organizations.