Artificial sweeteners determination - sucralose (HPLC)
From a health perspective, there is no harm in consuming foods and beverages prepared with artificial sweeteners that do not contain calories and do not provide energy. In fact, using artificial sweeteners is people's own choice. In order to use artificial sweeteners that do not lose weight because they do not provide energy, their feeding habits need to be controlled. There are two important points in weight control. The first is the control of energy intake by food, and the second is the regulation of physical activity.
Artificial sweeteners and other sugar substitutes are also used by diabetics as they satisfy the need for sweets without affecting blood sugar.
Sucralose is the latest artificial sweetener that was approved for use in Canada in 1991. Sucralose is obtained from sugar by chlorination method. 600 is sweeter than sugar. Sucralose, which does not contain metallic taste and which can come without any heat treatment, is known as the sweetener whose taste is most similar to sugar.
In recent years, it has been argued that artificial sweeteners, whose primary goal is not to lose weight, cause weight gain as opposed to known. In a study conducted on 1000 women in the United States, 79 revealed that women using artificial sweeteners gain more 7 percent weight in a year than non-users. Similar results were obtained in animal studies. This is explained in the following way. People who use artificial sweeteners meet the sweet need, but since the organism cannot get the necessary calories, then high calorie foods are taken.
In the context of chemical food analysis, studies are carried out by authorized laboratories to determine the sucralose substance among many artificial sweeteners. High performance liquid chromatography method (HPLC) is used in these studies and the standards published by local and foreign organizations are followed. The standard considered in the determination of sucralose is:
- TS EN 16155 Foodstuffs - Determination of sucralose - High performance liquid chromatography method