urease activity determination

Determination of Urease Activity
Determination of Urease Activity

Determination of urease activity in feeds is important in terms of feeding of animals. The rate of protein utilization decreases in animals consuming high urease activity. In our country, especially urease activity analysis is performed in mixed feeds added to soybean.

In laboratories, whether urease activity in mixed feeds is determined qualitatively by non-existent tests or urease activity is determined quantitatively. In quantitative urease activity studies, the urease enzyme in the feed sample is released from the buffered urea solution by ammonia titration method and urease activity is determined.

The urease enzyme catalyzes the hydrolysis of urea to carbon dioxide and ammonia. This enzyme is found in plant seeds and microorganisms. The urease in plants is located in the cytoplasm. In order to activate urease in bacteria, its subunits must be bound to the nickel ion. Urease enzyme is mostly found in soybeans. Urease is not harmful to humans, but fresh soybeans contain other unhealthy compounds.

The urease activity tests performed in the laboratory are performed to detect the urease enzyme. During the hydrolysis of urea by urease, a specific enzyme, ammonia and carbon dioxide are formed. Ammonia in the medium causes the pH to rise. Many methods have been developed for urease testing. Which one to choose depends on the researcher's choice.

Authorized laboratories conduct urease activity determination in compound feeds within the scope of chemical food analyzes. During these studies, the standards issued by national and international organizations are complied with. A few standards are based on:

  • TS 4707 ISO 5506 Soybean products - Determination of urease activity
  • TS 10338 Animal feed - Determination of urea
  • TS 6319 Animal feed - Urea molasses
  • TS 8477 Animal feed - Feeding urea