hydroxymethyl furaldehyde determination (Spectrophotometric)

Determination of Hydroxymethyl Furfurol (Spectrophotometric)
Determination of Hydroxymethyl Furfurol (Spectrophotometric)

The honey is heated, reducing its viscosity during processing and bottling. In this case, the glucose is made completely soluble and pasteurized. On the other hand, because of the high content of fructose in the honey content and the low pH of the honey, the heating process needs to be carried out very carefully. The content of honey in the fructose ratio is between 27-44 percent, the glucose ratio is between 22-40 percent, and the maltose ratio is between 3-16 percent. Under storage conditions the color of the honey darkens, the aroma intensity decreases and the amount of hydroxymethyl furfurol increases depending on the storage time and temperature. Therefore, determination of hydroxymethyl furfurol in honey is important. Increasing the amount of hydroxymethyl furfurol is undesirable in honeys.

According to the Turkish Food Codex Honey Communiqué (Communiqué no: 2012 / 2012) issued by the Ministry of Food, Agriculture and Livestock in 58, the amount of hydroxymethyl furfurol required in the honey content, flower honey, secretory honey and their mixtures cannot exceed 40 mg / kg. should be.

The higher the heat treatment and storage temperature applied to the honey, the higher the amount of hydroxymethyl furfurol. This causes changes in the taste, smell and color of honey. Honey must be quickly pasteurized to prevent the formation of hydroxymethyl furfurol. The amount of hydroxymethyl furfurol can be determined rapidly using a spectrophotometer.

In authorized laboratories, within the scope of chemical tests Determination of hydroxymethyl furfurol according to spectrophotometric method is carried out. In this method, hydroxymethyl furfurol is reacted with certain acids and the resulting color density read by spectrophotometer to quantify.

In these studies, the standards published by local and foreign organizations and the test methods accepted worldwide are complied with. The standard used to determine the amount of hydroxymethyl furfurol in honey is:

  • TS 13356 Determination of hydroxymethylfurfural content in honey - High performance liquid chromatography (HPLC) method