Bees take flowers and fruit buds and swallow the nectar, using an enzyme in their stomach to change the chemical and put it in the honeycomb cells in the hive. In the bees' stomachs, nectar is transformed into honey, thanks to the enzyme sucrose changes and turns into simple sugars in the form of fructose and glucose. The bees then blow some water to prevent fermentation. In this way, honey contains around 17 percent water, around 80 percent, mainly fructose (fruit sugar) and glucose (grape sugar) and small amounts of other sugars. The remainder are acids, proteins, ash and trace elements around 3. The higher the water content of honey, the more easily it deteriorates.

While obtaining honey, heat treatment is applied in order to facilitate filtration and delay crystallization of honey. This process causes a decrease in diastase activity. This indicates that honey has been added with external additives and that the honey has been heat treated above 55 degrees.

Diastasis in honey cannot be less than eight. However, the number of diastases cannot be less than three in honey, which naturally contains a low amount of enzyme, such as citrus honey. The number of disastases gives information about the enzymes in honey.

Diastase number determination in honey content is carried out in authorized laboratories within the scope of chemical tests. In this study, a certain amount of honey is mixed into a certain concentration of starch solution and kept at constant temperature. In this period, starch hydrolysis occurs with the diastase enzyme in honey. At the end of the experiment carried out under the required time and conditions, the remaining unhydrolyzed starch is converted to a colored complex with iodine solution and the different volumes of starch solutions are treated in the same process. In this way, the amount of starch solution to which 1 grams of honey can be fully hydrolyzed is calculated.

In these studies, the standards published by domestic and foreign organizations are complied with. The standards for determining the number of diastases are as follows:

• TS 3036 Honey