dye Matter analysis - Malachite green (LC-MS / MS)
Malachite green (or malachite green) is one of the toxic chemicals used in dyeing. When this compound is dissolved in water, it is a substance which prevents parasites, prevents fungal and bacterial infections and shows antiseptic properties, especially for marine organisms. It is generally used as a dye in the bacteriology, which examines the properties of all microbes and bacteria.
As a result of animal tests, cancer was detected in animals fed with malachite green. It has been determined that it causes some health disorders and liver tumors in people who eat seafood containing malachite green. Therefore, malachite green dyestuff was found to be carcinogenic.
Today, in some countries, malachite green is allowed to be used outside aquaculture as it is cheap and easy to use. For example, the American Food and Drug Administration (FDA) prohibited imports from China because malachite green was found in aquaculture imported from China. Subsequently, the use of this chemical component in aquaculture is prohibited in China.
Malachite chemical is used because it prevents a fungal disease that occurs in fish eggs. It is used as a dye when analyzing cells and culture samples in laboratories. It is also used as a pH indicator.
The Ministry of Food, Agriculture and Livestock aimed to make malachite green and leuko malachite green residue analyzes in fish in 2010 and LC-MS / MS chromatographic analysis method was developed within the scope of this project. This method is based on new analytical standards for residue monitoring introduced by the 2002 / 657 / EC directive.
In the laboratories, malachite green dyestuff analyzes are carried out in aquaculture within the scope of chemical tests and LC-MS / MS method is applied mainly. This method, called liquid chromatography - mass spectrometer, is a chemical analytical method that combines the mass analysis capabilities of the mass spectrometer with the physical separation properties of liquid chromatography.