Crystal violet dyestuff is a chemical dye which is used under microscope to see bacterial cultures which are colorless and transparent. When the correct staining method is applied, Gram negative bacteria appear in pink color. The Gram staining technique was developed by the Danish scientist Gram and is used to separate bacteria into two different groups according to the physical and chemical properties of the cell walls (Gram positive and Gram negative).

Because microorganisms are very small and colorless, it is very difficult to examine them under a microscope. It should be painted in order to facilitate this process and to obtain clearer images and accurate information. The microscopic sizes, shapes, arrays, cell structures and behavior of various dyes (such as Gram-positive, Gram-negative or acid-resistant bacteria) are determined only by staining.

For the purpose of microscopic examination, preparations are prepared from suspected cultures or disease-causing substances, and then different staining methods are applied depending on the sample taken and possible effect.

In fish farms, water is disinfected to protect fish and eggs from bacteria, fungi, viruses and many other parasites. This disinfection process previously used malachite green. However, after the prohibition of this chemical compound by legal regulations, crystal violet fungicide, which is an effective disinfectant, is now used. This compound is applied to farmed fish at a certain concentration and for a certain period of time.

 

In laboratories, within the scope of chemical tests, analysis of crital violet dyestuffs are performed in fisheries and LC-MS / MS method is generally used in these analyzes. Full description liquid chromatography - mass spectrometer This method is a chemical analytical method that combines the mass analysis capabilities of the mass spectrometer with the physical separation properties of liquid chromatography.