Not me Acid Analyses - methionine determination (HPLC)

Amino Acid Analysis - Determination of Methionine (HPLC)
Amino Acid Analysis - Determination of Methionine (HPLC)

Proteins, which are the most important nutrients in healthy and balanced nutrition of animals, amino acid chains. Some of the over twenty types of amino acids are essential and the human body does not produce them spontaneously. These essential amino acids include methionine. Since the body cannot produce essential amino acids, it must be taken through nutrition. Other amino acids are already produced by the body.

Proetines, which also contain methionine amino acids, are energy sources for animals. It is effective in terms of balanced nutrition and healthy development of their bodies. It is also necessary for the development of the skeletal system and tissues of animals. Proetines are involved in all chemical reactions necessary for life in the animal body. Proteins are also effective on the immune system of animals.

Methionine and cystine, which are among the basic amino acids, are highly effective on reproductive functions of animals, egg production and fertility. In particular, the amino acids cystine, lysine and threonine together with methionine are essential for animal development. Methionine and cystine amino acids are abundant in soybeans.

Among the amino acid analysis methods, HPLC derivatization method, which is a conventional method in the determination of methionine content in feed, is used. HPLC is a high performance liquid chromatography instrument and is widely used in chemical analysis. In this method, getting results is fast and effortless.

In the laboratories, methionine is determined among amino acid analyzes within the scope of chemical tests and the standards prepared by domestic and foreign organizations are followed in these studies. The standard used in these studies is as follows:

  • TS EN ISO 17180 Animal feed - Determination of lysine, methionine and thronine from commercial amino acid products and premixes