Proper nutrition is an important issue in animals as well as in humans. Animals need to have balanced development, be healthy, provide quality products and be able to fight diseases. Because this indirectly affects human health. Agricultural areas are decreasing and animal feeding costs are increasing. Therefore, there is a need for feeds that will be used less but which will yield more yield. Various microorganisms, growth accelerators, vitamins, enzymes, disease inhibitors and preservatives are added to the feeds.

There are five main nutrients: energy sources such as carbohydrates and fats, proteins, vitamins, minerals and water. Among these proteins are chemical compounds consisting of amino acid chains. They contain carbon, hydrogen, nitrogen and oxygen. Lysine is present in the basic amino acids. Amino acids have very important functions in the nutrition and development of animals.

In addition, lysine is one of the essential amino acids that people should take through nutrition. People need lysine between 1-1.5 grams daily. Lysine plays an important role in the synthesis of antibodies, enzymes and hormones by the body in the process of building muscle proteins, calcium absorption, healing after surgery or sports injuries.

Among the amino acid analysis methods, HPLC derivatization method, which is a traditional method, is used for determination of lysine content in feeds. HPLC is a high performance liquid chromatography instrument and is widely used in chemical analysis.

In the laboratories, lysine determination is also made between amino acid analyzes within the scope of chemical tests and the standards prepared by domestic and foreign organizations are followed in these studies. The standard used in these studies is as follows:

• TS EN ISO 17180 Animal feed - Determination of lysine, methionine and thronine from commercial amino acid products and premixes