The phenomenon of dense shellfish poisoning (DSP) was first encountered in Japan in 1976. However, these poisonings have probably been encountered before, but not as a Diarrhetic Shellfish Poison Group (DSP) but as a bacterial infection. These intoxications have attracted attention during the overproduction of dinophysis and prorocentrum algae species in recent years. Ocaic acid, produced by marine dinoflagellates, is a fat-soluble compound that causes sudden diarrhea in humans. In case of prolonged exposure to ocaic acid, there are also observations that they are carcinogenic.

Only ocaic acid groups have direct effects on humans. To date, no poisoning has been observed in humans due to pecototoxin and yessotoxin. In other countries, there is no active active value for yessotoxin and pektenotoxin.

The Regulation on Special Hygiene for Animal Foods issued by the Ministry of Food, Agriculture and Livestock describes the specific hygiene conditions that food enterprises producing animal food must comply with in addition to the principles laid down in the Food Hygiene Regulation. Accordingly, the total amount of marine biotoxins measured in the bodies or edible parts of shellfish must be at most:

• For the sum of ocadaic acid, dinophysis and pecteno toxins, 160 okg / kg as the equivalent of ocadaic acid
• 1 mg / kg equivalent of yesso toxins for yesso toxins
• 160 azg / kg as azaspiric acid equivalent for azaspiric acids

 

Azotpiric acid 2 (AZA-2) analysis is performed among Diarrhetic Shellfish Poison Group (DSP) intoxication analyzes by liquid chromatography - mass - mass spectrometry method within the scope of biotoxin analyzes in authorized laboratories. Briefly called LC-MS / MS, this method combines the mass analysis capabilities of the mass spectrometer with the physical separation properties of liquid chromatography.