The Regulation on Special Hygiene Rules for Animal Foods was published by the Ministry of Food, Agriculture and Livestock in 2011. This regulation describes the special hygiene conditions that animal food producing establishments must comply with the principles stated in the Food Hygiene Regulation. This regulation is based on the Directive 853 / 2004 / EC on the Specific Hygiene Rules of Animal Foods issued in the European Union.

The provisions of the Regulation include marine biotoxins, ie toxic substances deposited by shellfish mollusks fed with planktons containing toxins. The eleventh section sets out special requirements for live bivalve molluscs. Accordingly, the total amount of marine biotoxins measured in the bodies or edible parts of live bivalve molluscs should not exceed the following limits:

• 800 Felg / kg for paralyzed shellfish venom (PSP)
• 20 mg / kg as domoic acid for shellfish poison (ASP) which causes forgetfulness
• For the sum of ocadaic acid, dinophysis and pecteno toxins, 160 okg / kg as the equivalent of ocadaic acid
• 1 mg / kg equivalent of yesso toxins for yesso toxins
• 160 azg / kg as azaspiric acid equivalent for azaspiric acids

In advanced laboratories, Diarrhetic Shellfish Poison Group (DSP) intoxication and azaspiric acid analyzes are performed by LC-MS / MS method within the scope of biotoxin analyzes. This method, called liquid chromatography - mass - mass spectrometer, uses the mass analysis capabilities of the mass spectrometer and the physical separation properties of liquid chromatography.

The main analyzes carried out within this framework are as follows:

• Diarrhetic Shellfish Poison Group (DSP) - Azaspiric Acid 1 (AZA-1)
• Diarrhetic Shellfish Poison Group (DSP) - Azaspiric Acid 2 (AZA-2)
• Diarrhetic Shellfish Poison Group (DSP) - Azaspiric Acid 3 (AZA-3)

 

These analyzes are based on standards and test methods published by domestic and foreign organizations.