amnesic Shellfish Poison group (ASP) (LC-MS / MS) domoic Acid (A-f)

Amnesic Shellfish Poison Group (ASP) (LC-MS / MS) Domoic Acid (DA-f)
Amnesic Shellfish Poison Group (ASP) (LC-MS / MS) Domoic Acid (DA-f)

Amnesic shellfish poisoning (ASP) group takes this name from memory loss, which is one of the symptoms of poisoning. Amnesia means memory loss. Amnesia is the condition where memory experiences problems and is impaired. These toxins were first spotted when blue mussel poisoning occurred on an island on the eastern coast of Canada and several people died. Domoic acid is a type of amino acid that is soluble in water and soluble in the nervous system. When a high level enters the human body, in mild events approximately 3 then causes vomiting, nausea, abdominal cramps and diarrhea. In severe cases, however, it causes high pain, decreased sensitivity, dizziness, short-term memory loss and hallucinations. It can cause deaths in newborns, kidney patients and people with weakened immune systems.

Amnesic poisoning is mostly caused by shellfish such as mussels and clams. For domoic acid which is water soluble and resistant to high temperatures, cooking and freezing processes, the maximum limit accepted by humans is 97 µg / g by the directive 61 / 20 / EEC in European Union countries.

In advanced laboratories, Amnesic Shellfish Poison Group (ASP) intoxication analyzes and domoic acid (DA-f) analyzes in shellfish are performed by LC-MS / MS method within the scope of biotoxin analyzes. This method, called liquid chromatography - mass - mass spectrometer, is a chemical analytical method that combines the mass analysis capabilities of the mass spectrometer with the physical separation properties of liquid chromatography.

These analyzes are based on standards and test methods published by national and international organizations. The standard considered in this respect is:

  • TS EN 14176 Foodstuffs - Determination of domoic acids in mussels by HPLC